Membrane-enrichment PCR / NGS preprocessing

Membrane-enrichment PCR / NGS preprocessing

Concentrate first, amplify second—start from a higher-concentration sample. The enriched membrane can enter downstream lysis, PCR amplification or NGS library preparation without first eluting targets back into liquid, reducing transfer loss and keeping more target nucleic acid in the workflow.

Platform promise

Collect more. Lose less.

Membrane enrichment concentrates dispersed targets into a compact area and gives every downstream method a stronger starting sample.

Why it works

Why targets are easier to detect

Membrane enrichment retains dispersed cells, bacteria and small particles on a compact membrane surface. More target reaches the next step, while fewer elution, transfer and repeat-preparation operations mean fewer opportunities for low-abundance material to be lost.

Every avoided elution or transfer is one less opportunity to lose a low-abundance target.

Core advantages

  • Typical workflows concentrate targets from large-volume samples by approximately 20–100×

  • The enriched membrane enters lysis, amplification or library preparation without first eluting targets back into liquid

  • Fewer tube changes, elution and recovery steps preserve more low-abundance target material

  • Connect PCR, qPCR, digital PCR and amplicon-sequencing workflows

Applicable samples and scenarios

  • Water, culture media and method-compatible liquid samples

  • Low-abundance bacteria, cells and nucleic-acid-bearing particles

  • PCR, qPCR, digital PCR, amplicon sequencing and NGS preprocessing

Workflow

  1. Prepare the sample workflow illustration

    Step 1Sample pretreatment

    Prepare the sample

    Choose preparation conditions from sample volume, matrix and target type to create a stable filtration input.

  2. Concentrate on the membrane workflow illustration

    Step 2Enrichment

    Concentrate on the membrane

    Retain low-abundance cells, bacteria or small particles on the membrane. Typical workflows provide approximately 20–100× enrichment, depending on input volume and process conditions.

  3. Lyse directly from the membrane workflow illustration

    Step 3NGS preprocessing

    Lyse directly from the membrane

    Place the enriched membrane directly into the downstream lysis step. Targets do not need to be eluted from the membrane back into liquid first, reducing another source of transfer loss.

  4. Connect amplification and library preparation workflow illustration

    Step 4NGS preprocessing

    Connect amplification and library preparation

    Feed the lysate into the existing PCR, qPCR, digital PCR, amplicon-sequencing or NGS library-preparation workflow.

Recommended product configuration

Data and research evidence

20–100×

Start with a more concentrated sample

Typical membrane-enrichment range; the actual factor depends on input volume, membrane area and process conditions.

No elution

No return to liquid phase

Targets do not need to be recovered from the membrane into liquid before the membrane enters lysis or amplification.

Integrated membrane direct-PCR study

Multi-workflow

Connect existing detection methods

Connect PCR, qPCR, digital PCR, amplicon sequencing and NGS library-preparation workflows.

Direct filter-amplification study

Get a solution

Bring more of the target into your detection workflow

Tell us your sample, target and current detection method. We will recommend the membrane route, consumables and workflow connection.

Get a PCR / NGS preprocessing solution