Shadowless-membrane microscopy and fluorescence

Shadowless-membrane microscopy and fluorescence

Keep cells, bacteria and small particles concentrated on the shadowless membrane and move directly into microscopy or fluorescence observation, reducing loss and morphology changes caused by elution and transfer.

Platform promise

Collect more. Lose less.

Membrane enrichment concentrates dispersed targets into a compact area and gives every downstream method a stronger starting sample.

Why it works

Why targets are easier to detect

Membrane enrichment retains dispersed cells, bacteria and small particles on a compact membrane surface. More target reaches the next step, while fewer elution, transfer and repeat-preparation operations mean fewer opportunities for low-abundance material to be lost.

Every avoided elution or transfer is one less opportunity to lose a low-abundance target.

Core advantages

  • Observe targets directly on the membrane with less loss from elution and transfer

  • Reduce morphology changes caused by repeated handling

  • Connect bright-field microscopy, morphology and compatible fluorescence methods

Applicable samples and scenarios

  • Evaluation examples: cerebrospinal fluid, pleural or ascitic fluid, urine, water, culture media and other method-compatible liquid samples

  • Evaluation examples: cells, bacteria, fungi, parasite eggs and other small particles

  • Bright-field microscopy, morphology and compatible fluorescence workflows

Workflow

  1. Prepare the sample workflow illustration

    Step 1Sample pretreatment

    Prepare the sample

    Select pretreatment from the sample matrix and target characteristics so particles are evenly dispersed.

  2. Filter and concentrate workflow illustration

    Step 2Enrichment

    Filter and concentrate

    Pass the sample through the shadowless membrane so target cells, microbes or particles are retained in a small surface area.

  3. Prepare directly on the membrane workflow illustration

    Step 3Slide preparation

    Prepare directly on the membrane

    Move the enriched membrane directly into slide preparation, clearing or staining without first eluting targets back into liquid.

  4. Microscopy and fluorescence workflow illustration

    Step 4Microscopy

    Microscopy and fluorescence

    Observe concentrated targets by bright-field or fluorescence microscopy with more targets in each viewing area.

Recommended product configuration

Data and research evidence

On-membrane

Observe after concentration

Targets remain concentrated in a small membrane area, increasing target density within each observation field.

No elution

Reduce recovery loss

Compatible workflows do not require targets to be eluted from the membrane back into liquid first.

Fewer transfers

Simpler sample movement

Fewer tube changes, recovery steps and repeat slide preparations reduce losses of low-abundance targets.

Less disturbance

Preserve morphology

Less repeated centrifugation, resuspension and transfer helps preserve cell and particle morphology.

Get a solution

Bring more of the target into your detection workflow

Tell us your sample, target and current detection method. We will recommend the membrane route, consumables and workflow connection.

Get a microscopy and fluorescence solution