SOLUTION · PCR / NGS preprocessing
Membrane-enrichment PCR / NGS preprocessing
Concentrate first, amplify second—start from a higher-concentration sample. The enriched membrane can enter downstream lysis, PCR amplification or NGS library preparation without first eluting targets back into liquid, reducing transfer loss and keeping more target nucleic acid in the workflow.
EVIDENCE
Why targets are easier to find
Membrane enrichment concentrates dispersed cells, bacteria and small particles on a small membrane area so more targets enter the next step, while less elution, transfer and repeated slide preparation reduces the loss of low-abundance targets during handling.
20–100×
Start with a more concentrated sample
Typical membrane-enrichment range; the actual factor depends on input volume, membrane area and process conditions.
No elution
No return to liquid phase
Targets do not need to be recovered from the membrane into liquid before the membrane enters lysis or amplification.
Integrated membrane direct-PCR study ↗Fewer transfers
Reduce handling loss
Fewer tube changes, elution and recovery steps reduce loss of low-abundance targets.
Elution-free in-situ PCR study ↗Multi-workflow
Connect existing detection methods
Connect PCR, qPCR, digital PCR, amplicon sequencing and NGS library-preparation workflows.
Direct filter-amplification study ↗FIT
Samples and settings
- Water, culture media and method-compatible liquid samples
- Low-abundance bacteria, cells and nucleic-acid-bearing particles
- PCR, qPCR, digital PCR, amplicon sequencing and NGS preprocessing
Core advantages
- Typical workflows concentrate targets from large-volume samples by approximately 20–100×
- The enriched membrane enters lysis, amplification or library preparation without first eluting targets back into liquid
- Fewer tube changes, elution and recovery steps preserve more low-abundance target material
- Connect PCR, qPCR, digital PCR and amplicon-sequencing workflows
WORKFLOW
Workflow
-
01Sample pretreatment
Prepare the sample
Choose preparation conditions from sample volume, matrix and target type to create a stable filtration input.
-
02Enrichment
Concentrate on the membrane
Retain low-abundance cells, bacteria or small particles on the membrane. Typical workflows provide approximately 20–100× enrichment, depending on input volume and process conditions.
-
03NGS preprocessing
Lyse directly from the membrane
Place the enriched membrane directly into the downstream lysis step. Targets do not need to be eluted from the membrane back into liquid first, reducing another source of transfer loss.
-
04NGS preprocessing
Connect amplification and library preparation
Feed the lysate into the existing PCR, qPCR, digital PCR, amplicon-sequencing or NGS library-preparation workflow.
ENRICHMENT FIRST · DETECTION NEXT
Bring more targets
into the detection workflow
Tell us your samples, targets and current detection methods, and we will recommend a membrane route, consumables and how to connect the workflow.
- Increase target concentration
- Reduce avoidable transfer and elution loss
- Connect existing detection methods
- Support manual and automated workflows