SOLUTION · Microscopy and fluorescence
Shadowless-membrane microscopy and fluorescence
Keep cells, bacteria and small particles concentrated on the shadowless membrane and move directly into microscopy or fluorescence observation, reducing loss and morphology changes caused by elution and transfer.
EVIDENCE
Why targets are easier to find
Membrane enrichment concentrates dispersed cells, bacteria and small particles on a small membrane area so more targets enter the next step, while less elution, transfer and repeated slide preparation reduces the loss of low-abundance targets during handling.
On-membrane
Observe after concentration
Targets remain concentrated in a small membrane area, increasing target density within each observation field.
No elution
Reduce recovery loss
Compatible workflows do not require targets to be eluted from the membrane back into liquid first.
Fewer transfers
Simpler sample movement
Fewer tube changes, recovery steps and repeat slide preparations reduce losses of low-abundance targets.
Less disturbance
Preserve morphology
Less repeated centrifugation, resuspension and transfer helps preserve cell and particle morphology.
FIT
Samples and settings
- Evaluation examples: cerebrospinal fluid, pleural or ascitic fluid, urine, water, culture media and other method-compatible liquid samples
- Evaluation examples: cells, bacteria, fungi, parasite eggs and other small particles
- Bright-field microscopy, morphology and compatible fluorescence workflows
Core advantages
- Observe targets directly on the membrane with less loss from elution and transfer
- Reduce morphology changes caused by repeated handling
- Connect bright-field microscopy, morphology and compatible fluorescence methods
WORKFLOW
Workflow
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01Sample pretreatment
Prepare the sample
Select pretreatment from the sample matrix and target characteristics so particles are evenly dispersed.
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02Enrichment
Filter and concentrate
Pass the sample through the shadowless membrane so target cells, microbes or particles are retained in a small surface area.
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03Slide preparation
Prepare directly on the membrane
Move the enriched membrane directly into slide preparation, clearing or staining without first eluting targets back into liquid.
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04Microscopy
Microscopy and fluorescence
Observe concentrated targets by bright-field or fluorescence microscopy with more targets in each viewing area.
ENRICHMENT FIRST · DETECTION NEXT
Bring more targets
into the detection workflow
Tell us your samples, targets and current detection methods, and we will recommend a membrane route, consumables and how to connect the workflow.
- Increase target concentration
- Reduce avoidable transfer and elution loss
- Connect existing detection methods
- Support manual and automated workflows